Next Generation Sequencing (NGS)

Whole Genome Sequencing

Library prep is performed using either the TruSeq Nano or TruSeq PCR-free Library kits, and sequenced on the NovaSeq6000 to desired depth of coverage.
A variety of bioinformatics analysis pipelines are available.

Whole Exome Sequencing (WES)

Library prep is performed using Agilent SureSelect V5/6/7 kits, followed by sequencing on the NovaSeq6000 to desired depth of coverage.

The standard analysis pipeline utilizes BWA-GATK. In addition to the raw sequence data, customers would receive the alignment file, list of SNP/InDel, and QC report.

Whole Transcriptome Sequencing (WTS)

Library prep is performed using the TruSeq stranded mRNA or TruSeq Total RNA ribozero kit, depending on customer requirements. Sequenced on NovaSeq 150PE to desired depth of coverage.

Trimmed reads are mapped to the reference genome; transcripts are assembled and differentially expressed gene analysis will be done. Analysis includes the transcript locus, readcount and FPKM for each transcript. GO Analysis is conducted, and KEGG analysis is also available. Analysis report includes both excel/csv data as well as graphical plots, such as hierarachical clustering, multidimensional scaling, box plots, etc.

Epigenomics – Whole Genome Bisulfite Sequencing (WGBS)

Library prep is performed using the Accel-NGS Methyl-Seq DNA Library.

Analysis includes calculation of bisulfite conversion rate, mapping to reference genome, alignment QC, and methylation level calling. Differentially methylated CpGs analysis is conducted and a list of significant CpG is generated. This data is presented via graphical plots as well.

Shotgun Metagenomics

We offer a variety of shotgun metagenomics packages. We recommend that PE250 sequencing be used to sequence the complex mixture of genomes present, so that higher accuracy can be obtained in the bioinformatics analysis.
However, the customer may choose the lower specification of PE150 sequencing, which is cheaper but results might not be optimal.

Sequencing Only

We also provide sequencing only services, where customer prepares the library using any of their preferred kits. Examples include single cell sequencing of 10x Chromium libraries, WES using Roche NimbleSeq, etc.

Custom Sequencing Services

We are able to provide full services using library kits not listed above, or sequencing on other platforms such as HiSeq2500, HiSeq4000, MiSeq, NextSeq, Ion Torrent, PacBio Sequel, Oxford Nanopore PromethION.

To Order:

Email, call +65 6339 0927 to speak to a Macrogen representative or use our online contact form.