Standard Sequencing
Macrogen has extensive experience and automated systems to provide quality Capillary Electrophoresis Sequencing (CES) service. Standard Sequencing Service is one of our most popular services utilized by customers from 153 countries
Our professional R&D team and our customer-oriented support team would ensure that you will be highly satisfied by our services, and quality of the work.
Scope of Service
- Sequencing using the Applied Biosystems 3730xl DNA Analyzer
- High quality results with average read length of 1,050bp
- Real-time monitoring is possible from order receipt to delivery of the results
- Results provided within 24 hours of sample’s arrival
- Free basic analysis service provided (ex. homology search using BlastN)
- Free universal primer is provided
Standard Sequencing Single/Plate
Single Tube Sequencing
- Samples are prepared in individual tubes upon request
- Free re-sequencing service provided
96 well Plate Sequencing
- Samples are prepared in 96-well plates upon request
- Up to 6 types of primer can be used for 1 plate
- Competitive price
Additional Service
- PCR product purification (both single/plate available)
- Gel extraction
- Confirmation of concentration by gel running
Difficult Template Sequencing
Difficult template sequencing is designed to deliver the sequence data for DNA templates that have unusual structures hindering the sequencing.
Examples of difficult template sequencing:
- siRNA with hairpin structure
- Unusual secondary structure
- GC/GT/AT-rich
- Homopolymeric tracts (Poly A, PolyG)
- Repetitive regions
Improved high-quality results can be obtained as shown in the example below:
Fragment Analysis Service
Fragment Analysis encompasses a wide variety of genotyping, DNA profiling, and mutation detection techniques for medical, environmental, and agricultural research. Macrogen has accumulated experience and significant know-how in genomics, thus enabling us to provide high quality Fragment Analysis services.
Fragment Analysis Service (Genescan Service)
Fragment analysis is provides precise size information of your fragment (PCR product) marked by fluorescent label.
Service Types:
- Microsatellite instability (MSI)
- Amplified fragment length polymorphism (AFLP) analysis
- Terminal restriction fragment length polymorphism (T-RFLP) analysis
- Relative fluorescent quantization - Loss of heterozygosity (LOH), Aneuploidy assays, and Large chromosomal deletion detection
- Sequence-related amplified polymorphism (SRAP)
Features:
- The resulting data is provided as an FSA file (PDF and Excel files are also possible)
- Results are provided within 3 to 7 business days after samples arrive
- Customized service from PCR optimization to fragment analysis is available
Standard Marker Types
Customized Sequencing
Macrogen provides customized sequencing service for our customer’s research projects which makes us total genomic service provider. Please contact us for more information.
List of services:
- 16s /18S /ITS & 26S rRNA Full Sequencing
- Primer Walking
- Cloning
- SNP/Mutation Analysis
- One click sanger sequencing
- DNA barcoding service